Visit www.lifetechnologies.com Gateway® recombination cloning technology circumvents traditional restriction enzyme based cloning limitations, enabling you to access virtually any expression system in just a few simple steps. From cloning or sub-cloning of DNA fragments over protein expression to functional analysis, Gateway® cloning technology is applicable for a variety of research areas, for truly multidisciplinary scientific studies and get's you there faster than with traditional methods. ------------------ Audio transcript: The typical cloning workflow involves many steps, takes a considerable amount of time and even then success is not guaranteed. Imagine a cloning workflow which was fast, flexible, efficient, and delivered consistent results. Well welcome to Gateway Cloning Technology. Regardless of the desired end result—cloning or subcloning of DNA fragments, protein expression, or gene analysis— Gateway Cloning Technology gets you there faster than traditional methods. Here's how it works. First, set up the reaction by adding the starting DNA material, buffer and clonase enzyme to your microfuge tube. Next, incubate at room temperature for one hour. Then add competent cells to your mixture and allow 45 minutes for the transformation to take place. After transformation, plate the reaction and incubate overnight at 37 degrees celcius. It is that simple! Gateway Cloning Technology gives you: • Fast, one hour, room temperature cloning reactions with greater than 99 ...
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