Learn more at: www.lifetechnologies.com How to order a subcloning additional to a gene synthesis online? This tutorial video demonstrates the functions of the subcloning form within the GeneArt online order portal. You will learn how to clone your gene into a Life Technologies vector or into a custom vector. [Audio transcript:] This tutorial shows how easy it is to set up gene subcloning into your vector of choice on our online portal. Enter a name for the final plasmid. Then choose from our selection of Life Technology vectors. Cloning sites will be predefined, based on the cloning site information provided in the gene synthesis form. For more information about the gene synthesis form, see the tutorial "overview of functions". The portal will automatically do a cloning of your pre-defined gene into the selected vector. If required, please select the alternative option. If you require subcloning into a custom vector, choose that option from the list.Select the resistance marker and the copy number of the destination vector. Indicate whether the vector is stocked at GeneArt® or is a new vector which will need to be sent to us. Provide us the vector name and paste your vector sequence into the vector sequence field. Alternativley, upload your sequence here. Then start cloning. The portal will automatically do a cloning of your pre-defined gene into the selected vector. If required, please select the alternative option. Then confirm the cloning strategy. The "vector summary ...
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