YLEX Expression Kit (Cat. #: FYY201-1KT).wmv

YLEX Expression Kit based on INRA INAPG licensed patent* provides an easy approach for cloning and expressing a gene of interest in the yeast, Yarrowia lipolytica. Using this kit, high level of heterologous protein may be expressed intracellularly or be secreted from the cell into medium by selecting the supplied expression vector pYLEX1 or pYLSC1. Vectors provided in the YLEX contain a strong hybrid promoter carrying four tandem copies of upstream activator sequences (UAS1B) fragment from pXPR2 and a minimal pLEU2 fragment. Unlike the frequently used Yarrowia promoter (pXPR2), this stable hybrid promoter directs protein expression constantly without multiple influences by nutritional and environmental factors in medium. When a constructed plasmid with the hybrid promoter followed by a cloned gene of interest is linearized by the selected restriction enzyme, it becomes an expression cassette that can integrate into the Y. lipolytica genome by homologous recombination within the process of transformation. The successful transformants are ready for expression or secretion of recombinant protein depending on whether secretion signal appears on the plasmid. For more information, please visit www.yeastern.com * INRA (Institut National de la Recherche Agronomique) and INAPG (Institut National Agronomique Paris-Grignon

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